Laboratory efforts to cultivate noroviruses

Laboratory efforts to cultivate noroviruses.

Noroviruses (NoVs) are a leading cause of gastroenteritis worldwide and are recognized as the foremost cause of foodborne illness. Despite numerous efforts, routine cell cultures have failed to yield replicating NoV. This paper describes methods used to try to grow NoV in vitro in two laboratories. Cells (A549, AGS, Caco-2, CCD-18, CRFK, CR-PEC, Detroit 551, Detroit 562, FRhK-4, HCT-8, HeLa, HE...

Porcine Noroviruses Related to Human Noroviruses

Detection of genogroup II (GII) norovirus (NoV) RNA from adult pigs in Japan and Europe and GII NoV antibodies in US swine raises public health concerns about zoonotic transmission of porcine NoVs to humans, although no NoVs have been detected in US swine. To detect porcine NoVs and to investigate their genetic diversity and relatedness to human NoVs, 275 fecal samples from normal US adult swin...

INCAS Neurocognitive Laboratory Four Years of Intensive Efforts to Build up Experimental Understructures for Human Subjects in Iran

Shadow imaging efforts at MIT Lincoln Laboratory

The technique of shadow imaging allows one to take pictures of distant objects that are beyond the reach of the largest ground-based astronomical telescopes. The technique relies on the fact that stars are nearly ideal point sources and are conveniently located behind man-made deep space objects, such as satellites. As a space object passes in front of a star (an “occultation event”), the objec...

Are post-Ebola reconstruction efforts neglecting public health laboratory systems?

David Evans and colleagues (August, 2015)1 modelled how the loss of health-care workers to Ebola virus disease (EVD) might have affected infant, under 5, and maternal mortality in Guinea, Liberia, and Sierra Leone. Unfortunately, laboratory workers were not included in the health-care worker definition used in their model. Laboratory workers are a central part of an effective health system. In ...